Research

Current Research

My current research has been focused increasingly on student learning in microbiology.  We investigate student perspectives of the online learning environment, student use of primary literature in interdisciplinary science and humanities courses, and evaluate course based undergraduate research experiences.

This work is primarily focused on the microbiology program with colleagues and students  from our undergraduate courses: MB351 General Microbiology lecture, MB352 General Microbiology laboratory, MB360 At the Bench: Scientific Inquiry in Microbiology: At the Bench, Crowdsourcing Antibiotic Discovery, and MB200 The Fourth Horseman: Plagues that Changed the World.

Select Publications:

  1. MB352 General Microbiology Laboratory Manual                                                                AM Lee. Biology LibreTexts. 2021. [ebook] Available at: https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)
  2. Redesign of an Undergraduate General Microbiology Lab to Include Authentic Discovery-Driven Research on Cucumber Fermentations
    J Greenstein, B Ford, S Gove, F Breidt, AM Lee, ME Taveirne
    doi: https://doi.org/10.1101/356261.  2018. BioRxiv preprint.
  3. Redesigning plants for spaceflight and beyond: transfer of genes from Pyrococcus
    YJ Im, R Killens, A Lee, M Ji, C Lowder, AM Grunden, WF Boss
    2010. Gravitational and Space Research 23 (2).
  4. Functional analysis of tungsten metabolism in Pyrococcus furiosus  AM Lee. Dissertation. 2009.
  5. Lee 08 JPR Proteomics of Pyrococcus furiosus, a Hyperthermophilic Archaeon Refractory to Traditional Methods AM Lee, JR Sevinsky, JL Bundy, AM Grunden, JL Stephenson Jr.  2009.  Journal of proteome research. 8:8:3844-3851.
  6. Expression of Pyrococcus furiosus superoxide reductase in Arabidopsis enhances heat tolerance. YJ Im, M Ji, AM Lee, R Killens, AM Grunden, WF Boss
    2009. Plant physiology 151 (2), 893-904
  7. Bacteria of dental caries in primary and permanent teeth in children and young adults
    JA Aas, AL Griffen, SR Dardis, AM Lee, I Olsen, FE Dewhirst, EJ Leys, and BJ Paster
    2008. Journal of clinical microbiology 46 (4), 1407
  8. Production of a thermostable archaeal superoxide reductase in plant cells
    YJ Im, M Ji, AM Lee, WF Boss, AM Grunden
    2005. FEBS letters 579 (25), 5521-5526
  9. Diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients
    CE Kazor, PM Mitchell, AM Lee, LN Stokes, WJ Loesche, FE Dewhirst, BJ Paster
    2003. Journal of clinical microbiology 41 (2), 558
  10. Bacterial diversity in necrotizing ulcerative periodontitis in HIV‐positive subjects
    BJ Paster, MK Russell, T Alpagot, AM Lee, SK Boches, JL Galvin, FE Dewhirst
    2002.  Annals of periodontology 7 (1), 8-16
  11. Comparative molecular analysis of tongue bacteria in halitosis and in health.
    AM Lee, PM Mitchell, LN Stokes, CE Kazor, WJ Loesche, FE Dewhirst, BJ Paster
    2002. Journal of Dental Research. 81, A116-A116
  12. Bacterial diversity in odontogenic infections.
    LN Stokes, TR Flynn, PM Mitchell, AM Lee, NA Prince, L Martin, BJ Paster
    2002.  Journal of Dental Research.  81, A364-A364
  13. Bacterial population distribution in healthy subgingival plaque.
    PM Mitchell, AM Lee, ACR Tanner, FE Dewhirst, BJ Paster
    2002.  Journal of Dental Research. 81, A371-A371

Check out my google scholar page.

Images from bench research with undergraduates.

Various projects: isolation of antibiotic producers from the soil, isolation of Lactic Acid Bacteria (LAB) on MRS media from home fermented products, evaluating inhibition of biofilm formation of Streptococcus mutans (key cariogenic organism) by selected LAB strains.

Patch/patch assay on PDA with soil isolates and tester strains.

Patch/patch assay on PDA with soil isolates and tester strains.

Organic extract of antibiotic producer under UV light.

Organic extract of antibiotic producer under UV light.

Large zone of inhibition observed with LAB isolate from pickles.

Large zone of inhibition observed with LAB isolate from pickles.

 

JFK doing a microtiter dish biofilm assay.

JFK doing a microtiter dish biofilm assay.

Crystal violet stain of Streptococus mutans biofilm growth

Crystal violet stain of Streptococus mutans biofilm growth

JFK at Undergraduate Research Symposium, 2016.

JFK at Undergraduate Research Symposium, 2016.